Description. D 1 1. For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Open the SoftwarePro® Data Acquisition & Analysis software,. The absorbance of MPO was calculated using the SpectraMax M5 (Molecular Devices Corporation, San Jose, CA, USA) at 460 nm. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes 6. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. These all-inclusive assay kits are ready to use in assay validation or screening. LMax, SpectraMax ®, VersaMax™, VMax , EMax , UVMax, FlexStation®, and ThermoMax benchtop Microplate Readers. Cables. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid-The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. 1. Fluorescent Polarization: Solid black plates, as above. Catalog Number: M5. Sale. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplate. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. . Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. Plates were mixed for 2 min on an orbital shaker and then incubated at room temperature for 10 min. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR. DNA provides the same sensitivity as a spectrophotometer. The following document is intended to demonstrate setup of this instrument and provide representativeThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. Set the excitation at 531 nm and emission at 595 nm. 3. TR-FRET assay. The following document is intended to demonstrate setup of this. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax M5, Analyst HT or Analyst GT. Description 1. Choose from a three- (M3), four- (M4), or SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 26 0112-0115J ApplicationsofAbsorbance Absorbance-baseddetectioniscommonlyusedtoevaluatechangesincolororturbidity,. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. 5. Select the fluorescence option and read the total fluorescence. HEALTH AND SAFETY CONSIDERAIONSThe SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Microplate Reader Shelf. Table of Contents. Molecular Device SpectraMax M5 Plate Reader - Certified with Warranty. Italic and boldface type are used for emphasis. The SpectraMax M5/M5e & FlexStation 3 instruments use SoftMaxPro® Data Acquisition & Analysis Software. spr SpectraMax Plus 384. spr SpectraMax iD5 SpectraTest ABS2 v1. Note: for the THUNDER assay, performance of a SpectraMax M5e reader will be equivalent to that of a SpectraMax M5 reader. 1321N1, a human astrocytoma cell line, expresses. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. 4. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. SpectraMax Spectrofluorometers. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. Figure 2. 7. In other words, I carry out equilibrium folding/unfolding titrations of proteins and monitor the process using. Some luminescence applications, such as gene reporter assays, may require a luminometer with photon counting detection for greater sensitivity, such as the SpectraMax M5, Analyst GT or Analyst HT, or LMax II. (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. 190-1000. Wavelength optimization. We would like to show you a description here but the site won’t allow us. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M5 Multi-Mode Microplate Reader. With its 5-color monochromator detection head, MOLECULAR DEVICES SpectraMax M5 is the ideal choice for high-throughput applications, providing superior results in the analysis of a. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. The dual monochromator optics allow the widest range of applications to be utilized for bior. The IP-One assay can be used to characterize compounds that act on Gq-coupled receptors in either adherent or suspension cells. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Qualify the fluorescence performance of the SpectraMax . Download PDF versions of the SpectraMax M5 User Guides from this page. . These settings are also valid for the SpectraMax M3/M4, FlexStation® 3 Multi-ModeWe would like to show you a description here but the site won’t allow us. Description 1. Spectral Range. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Table of Contents. . +1 800-635-5577. Control Panel The control panel houses the instrument status LEDs and the microplate. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. Flexible for 6-well to 384-well microplates. 1. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Description 1. edu) to be added as a trained user in iLab. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. For the SpectraMax M3/M4/M5 readers - use the Purple Assay Plate adapter for Top Read only. Unit comes with software and computer. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Page: 1/6. Also refer to this Multimode Reader Comparison Chart. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. ) Click the "OK" button on SoftMax Pro Setup dialog box . Find many great new & used options and get the best deals for Molecular Devices SpectraMax M5 Microplate Reader with 90-Day Warranty at the best online prices at eBay! Free shipping for many products!Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Samples were measured together with a corresponding reference solvent contained in a. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. -Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. In the M2, M5 and Plus 384, the light. HEALTH AND SAFETY CONSIDERAIONSThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The integration time suggested is 5-10 sec. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, Gemini EM reader, SpectraMax M5/M5e reader, FlexStation 3 reader, SpectraMax M4 reader Which is better: top-read or bottom-read? You can use either. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. There were 12 replicate wells per dilution in both 96-well and 384- well plates. . SpectraMax M3, M4, and M5/M5e readers. Table of Contents. Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. SPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance (ANOVA) (Table 1). The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. com A. . It does not work as expected. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. The drawing shows a standard microplate of 15 mm height covered with a lid. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 305 reference level saturation. Two sequential measurements should be carried out: at 620 nm for the cryptate emission, and at 665 nm for the specific signal emitted by the acceptor. . By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. SpectraMax M Series multi-mode microplates are configurable readers with triple-mode cuvette ports, validation tools, and IQ/OQ. SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. The M5 has two PMTs. All reagents were brought to room temperature before use. Bookings are. The plastic from a microplate will affect the light polarization, precluding bottom reads and reading a covered plate. 190-1000 nm, tunable in 1 nm increments. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Currently we have over 300 Windows 7/10 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. Step 4. Refurbished. Open the Softmax Pro software and select the FP option. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. OptimizingAbsorbanceReadMode The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Data collected by Analyst® and FLIPR® Systems can also be imported and analyzed in the software. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The plate reader. Principle of the HTRF IP-One competitive binding assay. The following document is intended to demonstrate setup of this. The following document is. 4. For example, microplates containing different samples can be read using the The interval between reads was set at 15 s or 60 s, respectively. Italic and boldface type are used for emphasis. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. 2. Microplate Readers include the M3, M4,. Read fluorescence polarization at room temperature (RT, 19–25°C). Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. 2. The. Choose from a three- (M3), four- (M4), orAll general chemicals and solvents were commercially available and used as received. Description 1. 7°C (gradient heating design). 2. no. Select the fluorescence option and read the total fluorescence. . SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images were obtained with SU8010 field. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. D 1 1. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of. Table of Contents. Pick the best speed, sensitivity, and price for your needs. Features. Turn on the microplate reader. We would like to show you a description here but the site won’t allow us. . The time course of the cleavage reaction was monitored by loss of FRET (excitation, 450 nm; emission, 655 nm) with 15 reads/well and 4–5-s read intervals on a SpectraMax M5 plate reader (Molecular Devices). Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. 2. Description. 检测模式包括： • 紫外-可见光 光吸收 (Abs) • 荧光强度 (FI) • 化学发光 (Lum) • 时间分辨荧光 (TRF) • 荧光偏振 (FP) SpectraMax M5e 在 M5 基础上通过了 Cisbio HTRF 检测认证而增加了应用范围。. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. 1step assay with SpectraMax microplate readers. These all-inclusive assay kits are ready to use in assay validation or screening. Set the excitation at 531 nm and emission at 595 nm. 1. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. Step 4: Incubate at room temperature for 1 hour, protected from light. . Statistical analyses The statistical analyses were performed using Graph Prism version 7. ) Turn on the SpectraMax L and connect the computer to the SpectraMax L using the USB cable. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The user manuals cover installation, troubleshooting, and validation of the SpectraMax M5 and its accessories. Choose cables that meet the requirement described in this appendix. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96-well black plates. Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. SpectraMax L Microplate Reader provides medium to high throughput. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. The BenchCel Microplate Handling Workstation provides microplate-orientation sensing so all microplates enter the reader in the same orientation, ensuring that the data output is consistent from the first microplate to the last. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. For more information, please visit us at Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Choose from a three- (M3), four- (M4), or Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Spectral Range. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. SpectraMax® Microplate Readers and SoftMax® Pro Software. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Wavelength optimization. Molecular Devices has collaborated with various assay partners to optimize and validate homogeneous and heterogeneous biochemical- or cell-based assay performance on the SpectraMax Microplate Reader platform. The Spectramax performs spectral scanning in 1 nm increments, and up to six wavelengths. Molecular Devices Spectramax M5 luminometer: Krishnamurthy lab-Praveen (lab WO3213, x4605) Spectramax plate reader: yes Beckman LS6000IC Scintillation counter: Bill Taylor (office WO4262B, x1966) WO3242 liquid scintillation counter Department backup -80oC freezer: Song-Tao Liu (WO4254, x7857) Facilities Facility: Department. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. If you do not have an iLab account you must first make one: SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability and also uses patented PathCheck® Pathlength Measurement Technology – the only temperature independent pathlength correction for microplates. The KeySpan USA-19HS is often required with new Windows 7. Sensitive fluorescent quantitation of DNA with the Quant-iT. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. 306 plate air cal fail,low light. Mass spectra were recorded using an HPLC-System Series 1100 coupled with ESI-single. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The Gemini XPS Reader has dual monochromators that allow users to utilize new and novel dyes without purchasing expensive filter sets. The test solution was by diluting FCV to PBS/ Glycerol solutions (5 μM, 1. . VWR offers biophotometers for your lab's needs. Description 1. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Wide range of applications. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). Optimal instrument settings are the same for all three readers. Description 1. Detection modalities are shown in Table 1-1. $4,499. Description. . SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). . The optical performance is comparable to a top-of-the-line dedicated The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. The following document is intended to demonstrate setup of this instrument. TR-FRET assay principle. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Other technologies such as imaging. Access this by going to Help >. Optimized for fluorescence microplate readers. STAKMAX. In the M2, M5 and Plus. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. 1. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. SpectraMax® M5/M5e/FlexStation® 3 Instrument Settings Instrument Wavelength Settings EXC Wavelength 575 nm EMS Wavelength 620 nm Auto Cutoff 610 nm Optimized SpectraMax® M5/M5e Settings Readings 10-50 PMT Auto Auto Calibrate On Transcreener® HTS is a universal, high throughput biochemical assayUser guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Exhibit with us {{>currencyLabel}} Back. . . 7°C (gradient heating design). It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. D 1. 314 low lamp level warning. The day after, a final concentration of 1 μmol/L ARS1620, 20 nmol/L SMARTpool siRNA or the combination of the 2 was added in 5 replicates (wells). The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 3. Dual monochromators provide the ability for. When the correct COM port is selected, the. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Spectramax M5 plate reader (96/384 format) Multimode photometrics for endpoint or kinetic measurements. These robust readers have been placed in labs from Antarctica to the. Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Published Dec 16, 2020 | Updated Dec 16, 2020. Wavelength Range. SpectraMax M5 microplate reader. NFkB Translocation is Proteasome-Dependent The proteasome is a large protein complex that degrades proteins into short peptides in a ubiquitin-dependent manner. SoftMax® Pro Microplate Data Acquisition and Analysis Software serves as the user interface to the microplate reader. free shipping. Description 1. Microplate Reader Shelf. Absorbance measurements of ADMA were acquired using a Biotek Cytation 5-plate reader. 5. Typical LLDs were approximately 1–2 fg/well in the Analyst GT and 2–3 fg/well in the SpectraMax M5. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes. 313 reference gain check fail. The Spectramax can accommodate cuvettes or a variety of 6 to 96-well plate formats. 303 unable to cal dark current. Lift up the lid over the chamber to insert or remove a cuvette. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. After 4 h, the fluorescence intensity of serum FITC-dextran was obtained at 480 nm excitation wavelength and 520 nm emission wavelength using a Spectramax M5 microplate reader (Molecular Devices). We would like to show you a description here but the site won’t allow us. used. Settings not required by a reader are indicated by ‘---’. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. SpectraMaxM3,M4,M5,andM5eMicroplateReaderUserGuide 26 0112-0115J ApplicationsofAbsorbance Absorbance-baseddetectioniscommonlyusedtoevaluatechangesincolororturbidity, permittingwidespreaduseincludingELISAs,proteinquantitation,endotoxinassays,and cytotoxicityassays. Add to Cart. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Examples: “Press carefully to engage,” “ Do not. Step 1: Open the SoftMax Pro IMAP FP protocol. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Consistent results are obtained over time offering workflow flexibility. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Download PDF versions of the SpectraMax M5 User Guides from this page. deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an. For example, microplates containing different samples can be read using the same orOn SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. 00 USD. A preconfiguredAcme Revival: Long Live DevicesWe would like to show you a description here but the site won’t allow us. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. ISM Equipment Classification (Group 1, Class A) This equipment is. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax Microplate Stacker for walk-away processing. UV/Vis Photometric Performance:. Our absorbance plate readers feature our PathCheck Sensor technology and. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. 0 mL). SoftMax Pro. 316 grating motor driver faulty. SoftMax Pro 5 GxP Software provides all the data collection and analysis tools of the standard edition, along withMolecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Kinase Binding and Adapta™ Europium-based TR-FRET assays. Wavelength Range. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Category 2 and 3 microplate readers use gradient heating as well. you can use a resource. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideIn this application note, we demonstrate the measurement of luciferase expression in CHO-K1 cells using the SpectraMax Glo Steady-Luc Reporter Assay Kit, which affords long-lasting luminescence signals. Note: this assay can also be detected using the luminescence mode of SpectraMax M5/M5 e and FlexStation 3multi-mode microplate readers. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Instructions on the proper use of the SpectraMax M5 Plate Reader. Monday to Friday 7am - 5pm Pacific Time. 5. 多功能酶标仪 FlexStation3 快速动力学操. $1,250. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Molecular Devices SpectraMax M5 Multimode Microplate Reader. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96. HTRF is a proprietary time-resolved fluorescence technology that overcomes The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading.